Furthermore, female C57BL/6 mice gain less weight, have improved insulin sensitivity, later onset of glucose intolerance, and a reduced inflammatory response (macrophage infiltration and inflammatory gene expression) in subcutaneous and gonadal adipose tissue compared to males during short-term (<20 weeks) HFD-feeding [129,130]; however, some (but not all) of these differences can be diminished or equalized by extending the amount of time spent on HFD and by using diets containing very high percentage of total and saturated fat. This evidence concerns the gene INS and Glucose intolerance.