Concordant with these changes in protein expression, KPT-185 treatment upregulated the classical p53 target p21 mRNA only in wt-p53 bearing Z138 and JVM2 cells but not in mt-p53 MINO and Jeko-1 cells (fold change; 2.2 for Z138, 3.1 for JVM-2, 1.2 for MINO, 1.3 for Jeko-1), and induced PUMA mRNA in all tested MCL cells irrespective of p53 status (fold change; 2.5 for Z138, 3.3 for JVM-2, 3.3 for MINO, 2.6 for Jeko-1) as assessed by PCR using TLDAs. This evidence concerns the gene CDKN1A and mantle cell lymphoma.