CSCs revealed a several-fold increase in PFKFB3 expression compared to cancer cells, which was correlated with downregulation of PFK1. It is interesting that PFKFB3 expression in all three types of breast CSCs is comparable, regardless of the heterogenous nature of breast cancer, which is indicated here by differential expression of CD24 [45]. This evidence concerns the gene PFKFB3 and breast cancer.