The results showed that heat shock protein 70 (HSP 70), A disintegrin and metalloprotease with thrombospondin motifs 1 (ADAMTS1), Beta-catenin as well as phosphorylated proline-rich Akt substrate of 40 kDa (PRAS40), WNK Lysine-deficient protein kinase 1 (WNK1) and Signal transducer and activator of transcription 3 (STAT3) were up-regulated in high-glucose treated HNSCC cells. The gene discussed is STAT3; the disease is head and neck squamous cell carcinoma.