POU5F1 and Hyperglycemia: Interestingly, there are no significant changes in CIC populations determined by aldehyde dehydrogenase activity (ALDH) [37] and Oct4 mRNA expression in HNSCC cells cultured in differential glucose environments (Supplementary Figure S2) indicating hyperglycemia facilitated cisplatin resistance in whole HNSCC cells rather than solely in HNSCC-CICs.