Herein, we found that miR-1207 activated the Wnt/β-catenin pathway in different layers through direct downregulation of SFRP1, AXIN2, and ICAT, hence enhancing ovarian CSC-like traits, demonstrating a novel mechanism on how Wnt/β-catenin signaling modulates CSC properties and might be widespread application among other spectrum of cancers. Here, CTNNBIP1 is linked to cancer.