VGF and type 2 diabetes mellitus: To confirm the mRNA expression data derived from AT and pancreas with and without T2D, we measured five proteins SYT4, VGF, BAG3, ApoA1, and VAV3 in four different groups of 41 human subjects; 1- Lean (control), 2- Obese, 3- Obese with T2D without receiving insulin (obese+T2D-INS), and 4- Obese with T2D receiving insulin (obese+T2D+INS).