In order to study the role of the EWS/FLI1 oncogenic transcription factor in maintaining super-enhancers specifically in Ewing sarcoma, we set up a Mir30-shRNA-mediated doxycycline-inducible EWS/FLI1 knockdown system in the Ewing sarcoma cell line TC32. This evidence concerns the gene EWSR1 and Ewing sarcoma.