In gastric cancer tissues, TEM1 expression was observed in none of the nine formalin-fixed, paraffin-embedded (FFPE) tissues when examined using immunohistochemical staining; however, TEM1 expression was detected in all seven frozen tissues using immunohistochemical staining, suggesting that TEM1 expression might be detected in the stromal cells of gastric cancer tissue samples in cases where the antibody was optimized for immunohistochemical staining using FFPE tissues 14. The gene discussed is CD248; the disease is gastric cancer.