First, the SERPINE1 knockout cell line in S26 (S26-SERPINE1−/−) was created through TALEN technology (Figures 6A–6C), and ectopic of SERPINE1 can rescue the abilities of migration and invasion in S26-SERPINE1−/− cells (Supplementary Figure S4), indicating that SERPINE1 is really crucial for cell migration and invasion in NPC. The gene discussed is SERPINE1; the disease is nasopharyngeal carcinoma.