The present study demonstrated that TGF-β1 markedly induced integrin αvβ3 expression, and blocking integrin αvβ3 by RNAi abrogated FGF1- and TGF-β1-induced wound healing in MCF10A cells as well as in a breast cancer cell line that naturally expresses moderate level of integrin αvβ3. Here, FGF1 is linked to breast cancer.