To validate MYLK and TKS5 as direct miR-200c target genes, we first analyzed endogenous expression patterns in epithelial luminal type cell lines T47D and MCF7 with high miR-200 expression levels versus more mesenchymal basal type breast cancer cell lines Hs 578T and MDA-MB-231 exhibiting only very low miR-200 levels. The gene discussed is MYLK; the disease is breast cancer.