We also identified one pathogenic variant in TP53 associated with Li-Fraumeni syndrome, which has an estimated prevalence of 1/5,000-1/20,000 and is characterized by several classic tumors, including soft tissue sarcomas, breast cancer, brain tumors, adrenocortical carcinomas, and leukemias [40]. Here, TP53 is linked to Li-Fraumeni syndrome.