IFNG and amyotrophic lateral sclerosis: As shown in Fig 3D, serum IFN-γ levels were markedly increased in both the ALS<12 m (262.82±30.91 pg/ml) and ALS≥12 m (289.34±17.48 pg/ml) subgroups compared with non-ALS patients (136.42±14.79 pg/ml, P<0.05), but no difference was observed between these two ALS patient subgroups (P>0.05).