Furthermore, as shown in Fig 2D, the serum IFN-γ levels were markedly increased in the bulbar (289.57±31.98 pg/ml) and limb (277.32±17.77 pg/ml) onset subgroups compared with the non-ALS patients (136.42±14.79 pg/ml, P<0.01), but no difference was observed between the bulbar and limb onset subgroups (P>0.05). The gene discussed is IFNG; the disease is amyotrophic lateral sclerosis.