GPC3 and hepatocellular carcinoma: Moreover, we pre-incubated HCC cells with HS20 before HGF treatment and found that HS20-treated HCC cells showed decreased levels of phosphorylated c-Met compared to control IgG-treated groups in Hep3B cells and Huh-7 cells, but not GPC3-negative SK-hep1 cells (Fig 5B).