ANXA5 and glioblastoma: Although A172 and U87 show a clear dose-dependent reduction in cell viability upon treatment with DMC, this is not accompanied by an enhancement of Annexin V or PI staining in these two cell lines (Figure 3B), the activation of caspases (Figure 4B) or the appearance of nuclear condensation (Figure 5), indicating that DMC did not trigger apoptosis or necrosis in these GBM cells after 24h incubation using the mentioned concentrations.