The protein level of SIRT1 was increased in L02 cells transfected with the miR-34a inhibitor compared with the cells transfected with the NC duplex (Fig. 3C) and the expression of SIRT1 was increased in HFD-induced hepatic steatosis mice treated with the miR-34a inhibitor compared to the HFD-induced hepatic steatosis mice treated with the NC duplex (Fig. 3D). Here, SIRT1 is linked to fatty liver disease.