BRAF and neoplasm: To validate the results obtained with Idylla and cobas, we subjected the 60 FFPE tumor samples (45 with wild-type BRAF and 15 with BRAF V600 mutations as per Idylla) to mutation analysis using the MiSeq deep sequencing system, in which the BRAF V600–surrounding region was amplified by PCR and sequenced at high depth (coverage >5000x) to facilitate the identification of mutations present in at least 1% of the DNA.