As Pim-1, another target of miR-33a, is up-regulated in NSCLC cells and down-regulation of Pim-1 by miR-33a may contribute to the invasion and metastasis in vitro12, 14, we compared the Pim-1 levels in NCI-H1299 cells with and without miR-33a replacement, and in SPC-A-1 cells with and without anti-miR-33a. The gene discussed is PIM1; the disease is non-small cell lung carcinoma.