NEK8 and nephronophthisis: Due to the localisation of both ANKS3 and ANKS6 to the primary cilium in the mouse kidney [7]and in Zebrafish [17], and experimental evidence ofANKS3 interaction with nephronophthisis proteins [4, 17], we tested the effects of in vivo Anks3 knock down on renal expression of genes encoding proteins present in the basal body (Cep290, Nphp5) and the transition zone (Nphp1, Nphp2, Nphp4) of the primary cilium, and in the ciliary axoneme (Nek8, Gli2) [18, 19](S3 Fig).