CSF2 and neoplasm: DC vaccines were obtained from bone marrow-derived DC precursors which underwent differentiation in the presence of GM-CSF and IL-4 for 6 days and then were loaded with tumor associated antigens in the form of tumor cell lysate or total tumor-derived RNA complexed with commercially available Lipofectamine 2000 (here and after LF) or with 2D3-DOPE cationic liposomes (here and after 2D3) [40].