Since analysis the level of Th1- and Th2-specific cytokines in blood plasma of mice with metastatic melanoma after DC vaccination did not let to determine exactly the type of Th response we studied the expression of master transcription factors Tbet (Th1), GATA3 (Th2), RORg (Th17) and Foxp3 (Treg) by real time RT-PCR in spleen cells of mice received DC vaccines (Fig 6). This evidence concerns the gene TBX21 and metastatic melanoma.