HSPA4 and cancer: To further confirm above notion in RY-2f mediated anti-cancer effects, we transfected A2780, HEY and OVCA433 cells with a plasmid containing constitutively active AKT1 (myr-AKT1) or control vector, respectively (Figure 5B), and then determined the anti-proliferative effect of RY-2f on the transfected cells.