We found that the total proportion of Annexin V positive cells, including Annexin V+/PI− (the right lower quadrant, representing early apoptosis) and Annexin V+/PI+ (the right upper quadrant, representing late apoptosis and necrosis) cells, were dose-dependently increased with the raising concentrations of RY-2f in all three ovarian cancer cell lines (Figure 3A and 3B). The gene discussed is ANXA5; the disease is ovarian cancer.