To study the impact of CFZ treatment on the expression of HLA on MM cells, we used flow cytometric analysis to test MM cell lines (KMS11, H929, RPMI8226, OCI-MY5, OPM2, ARK, U266, ARP-1, and RPMI8226/R5) by gating annexin V and 7AAD double-negative cells. The gene discussed is ANXA5; the disease is Miyoshi myopathy.