In the current study, using qMSP assay, we found that ZNF671 methylation exhibited a sensitivity of around 45% (42%-48%) in detecting bladder UC in urine, and that combining methylated ZNF671, IRF8 and sFRP1 increased the overall sensitivity for cancer detection in urine, to 96.2% (overall disease), 90% (low-grade disease) and remarkably 100% for high-grade tumors (Table 5). Here, SFRP1 is linked to cancer.