Although ALL cell lines expressed ligands for the DNAM1 receptor, i.e., Nectin-2 for all three cell lines and PVR for Nalm6 (Figure 5C), the addition of a blocking antibody against DNAM1 did not reduce the cytotoxic activity of activated NK cells against any of the three ALL cell lines (Figure 5D). This evidence concerns the gene CD226 and acute lymphoblastic leukemia.