In spite of the lack of statistically significant differences among the total enzyme activity when separated by tumor size, the tendency is clearly observed (P = 0.068 for total HK, Figure 3A and 0.057 for total PFK, Figure 3C; two-way ANOVA), and the increase is supported by the particulate enzymes activity results (Figure 3B and 3D). Here, HK1 is linked to neoplasm.