Through in vitro studies, we found that both transfected miR-146b-5p and TRAF6 siRNA significantly reduced the Ki-67 expression (P < 0.05∼0.001) and proliferation (P < 0.05∼0.001) of glioblastoma cell lines, as measured by Western blotting (Figure 4A and 4B) and CCK8 proliferation assay (Figure 4C and 4D). This evidence concerns the gene MKI67 and glioblastoma.