As shown in Figure 5, phosphorylated TAK1 and IκBα (P-TAK1 and P-IκBα) were significantly reduced in three glioblastoma cell lines transfected with miR-146b-5p or TRAF6 siRNA as compared with mock and negative control ones (P < 0.01 ∼ 0.001; Figure 5A–5C), but the expressions of TAK1 and IκBα remained constant among four groups of the three cell lines (P > 0.05; Figure 5A, and 5E). The gene discussed is TRAF6; the disease is glioblastoma.