By this method, we determined the distribution of CTCs classified by the expression of EpCAM in 46 NSCLC patients with stages from I to IV, investigated their diagnostic value in systematic therapy by longitudinal monitoring of these CTCs in 4 index patients, assessed the prognostic value of these CTCs and characterized the stemness of these CTCs by measuring the expression of CXCR4 and CD133. The gene discussed is PROM1; the disease is non-small cell lung carcinoma.