The tissues included triple negative breast cancer n = 41, HER2+ breast cancer n = 15, ER+HER2- breast cancer n = 8, pancreatic adenocarcinoma n = 150 (including 30 liver metastases), colorectal carcinoma n = 68, and glioblastoma n = 44. The gene discussed is ESR1; the disease is glioblastoma.