Consistent with a previous report demonstrating that p25 overexpression is associated with increased BACE1 mRNA, protein, and activity in human neuroblastoma SH-SY5Y cells [20], BACE1 protein levels, BACE1 activity, and Aβ secreted in the medium were increased substantially in PC12 cells stably transfected with p25 (Fig 2A and 2B). This evidence concerns the gene BACE1 and neuroblastoma.