To further assess the role of Thr252 phosphorylation in BACE1 enzymatic activity, SK-N-BE(2)C human neuroblastoma cells were transfected with either a control plasmid or with plasmids encoding full-length wild-type BACE1 (WT) and a phosphorylation-defective BACE1 mutant (T252A). The gene discussed is BACE1; the disease is neuroblastoma.