It was previously reported that expression of lncRNA-HEIH was decreased after knockdown of Sp1 by RNAi in HepG2 and Huh7 cells [16] and therefore, we initially used RNAi to investigate the role of Sp1 in regulating a panel of lncRNAs identified in HCC cell lines. The gene discussed is SP1; the disease is hepatocellular carcinoma.