The antidiabetic drug metformin [33] decreases expression of Sp1, Sp3, Sp4 and Sp-regulated genes in pancreatic cancer cells [18] and metformin (0–20 mM) inhibited growth of HepG2, SNU-449 and SK-Hep-1 cells after treatment for 24 and 48 hr (Fig. 7A). This evidence concerns the gene SP4 and pancreatic neoplasm.