Using knockdown of Sp1 (siSp1) by RNA interference (RNAi) as a model, we initially investigated the effects of loss of Sp1 on expression of lncRNAs highly expressed in HCC cells, namely HULC, lncRNA-HEIH, AY129027, DQ786243 and HOTAIR [5] (Fig. 1C). The gene discussed is SP1; the disease is hepatocellular carcinoma.