We carried out shRNA-mediated LDOC1 knockdown in the untransformed CGHNK6 and CGHNK2 cells to examine the tumor suppressor activity of LDOC1. Reduction in LDOC1 expression led to acquired anchorage-independent growth of CGHNK2 cells whereas no colonies grew by the knockdown of LDOC1 in CGHNK6 cells (Figure 5C). This evidence concerns the gene LDOC1 and neoplasm.