SOX9 and chondrosarcoma: Thirdly, using human chondrosarcoma cell line SW1353 transfected with miR-494 mimics, we validated that SOX9 was downregulated at mRNA and protein level, which was further confirmed by the luciferase reporter assay, suggesting that miR-494 directly binded to SOX9 3′-UTR in chondrosarcoma cells.