NDRG2 and colorectal cancer: Consistent with the inhibition effect of NDRG2 overexpression on glycolysis and glutaminolysis, knockdown of NDRG2 by lentivirus-mediated shRNA in colorectal cancer cell lines (Figure 1D and Supplementary Figure S3A) facilitated glycolysis and glutaminolysis, as indicated by increased glucose consumption and lactate production in Caco-2, HT-29 and HCT116 cells (Figure 1E and Supplementary Figure S3B), increased glutamine consumption, glutamate concentration in the culture medium and intracellular glutamate concentration in HCT116 cells (Figure 1F and Supplementary Figure S3C).