From more than 3,000 hybridoma clones, we identified 49 anti-EpCAM mAbs, five of which possessed high binding activity to several human cancer cell lines (SAS, NPC, HCT116, H441, MCF7, BxPC-3, and SKOV-3), but not normal cell lines (HUVECs and NNM). The gene discussed is EPCAM; the disease is cancer.