When the functionality of splenic immune cells from tumor bearing mice was examined, it was observed that 4T1-bearing mice treated with MVA-TWIST/TRICOM exhibited improved CD4+ T-cell proliferation and CD8+ T-cell secretion of IFN-γ in response to Twist peptide compared to untreated animals (Figure 3A and 3B). This evidence concerns the gene CD8A and neoplasm.