In order to determine if tissue source and obesity impacted adipogenic programming, expression of adipocyte (adipsin, leptin, GLUT4), lipolysis (ATGL) and key adipogenesis regulating transcription factor (PPAR-γ) were profiled in ASC at baseline and following 14 days of adipocyte induction (Fig 2D). Here, CFD is linked to obesity due to melanocortin 4 receptor deficiency.