TNF and stroke disorder: We surveyed the gene expression of a number of cytokines and chemokines—CCL2, CCR2, CCL5, CXCL10, CXCR3, IFNγ, TNFα, IL1β and IL6—in the ischemic cortex at 3 days post-stroke, but did not find statistically significant differences between mutants and controls (S4A Fig), thus future studies on protein levels or spatial distribution will be required to determine potential differences in the cytokine profiles between the two groups.