TNF and adenocarcinoma: Both CO and NAC have been reported to influence the expression levels of TNF-α.[2, 23] Treatment of the adenocarcinoma cell line Caco-2 with NAC-CORM showed a synergistic effect promoting a substantial inhibition of the expression of endogenous TNF-α at 4 and 12 h when compared with both CORM-3 and NAC alone at the same concentration (150 μm), as measured by enzyme-linked immunosorbent assay (ELISA) (Figure 4 B).