Western blot and IF analysis revealed that overexpression of miR-424 in HCCLM3 decreased the expression of vimentin and increased the expression of E-cadherin in HCC cells, whereas inhibition of miR-424 in SMMC7721 increased the expression of vimentin but decreased the expression of E-cadherin (Fig S4A & S4C). This evidence concerns the gene VIM and hepatocellular carcinoma.