NPM1 and anaplastic large cell lymphoma: As a model system to develop and test hiBA-FISH, we designed probes for the well-characterized recurrent translocation between the 5’ region upstream of the NPM1 breakpoint in intron 4 (chromosome 5q35) and the 3’ region downstream of the ALK breakpoint in intron 19 (chromosome 2p23) in anaplastic large cell lymphoma (ALCL) [17].