However, further studies are needed to confirm whether the activation of MAPK or AKT pathways is required for CCDC34-mediated proliferation and migration, e.g. the effect of CCDC34 knockdown plus blocking the MAPK or AKT pathway on bladder cancer cells proliferation, apoptosis and migration; and how CCDC34 regulates the phosphorylation and gene expressions. This evidence concerns the gene AKT1 and urinary bladder cancer.