To enhance the anti-tumor activity of PBLs expressing EpCAM specific CAR against the immunosuppressive PC3M tumor, we generated CAR-Akt, or CAR-myr-Akt co-expressing retroviral vectors (Fig. 4b), and verified the transduction efficiency of CAR and Akt in PBLs by RT-PCR, real-time PCR and western blot, respectively (Fig. 4c). The gene discussed is AKT1; the disease is neoplasm.