Prostate cancer cell line PC3M highly expresses EpCAM, and human peripheral blood lymphocytes (PBLs) were engineered to recognize PC3M by transducing with EpCAM specific chimeric antigen receptor (CAR) which consists of an anti-EpCAM scFv, part of the extracellular domain and the entire transmembrane and intracellular domains of CD28, and the cytoplasmic domain of CD3ζ. The gene discussed is CD28; the disease is prostate cancer.