To investigate if transducing wtAkt or myr-Akt could improve the therapeutic effect of adoptively transferred T cells against tumor, C57BL/6 mice were inoculated with B16-OVA tumor cells subcutaneously, when tumor size reached ~6 mm, in vitro stimulated and expanded OT-1 cells transduced with control retroviruses or retroviruses encoding wtAkt or myr-Akt were intratumorally injected into mice. The gene discussed is AKT1; the disease is neoplasm.