In contrast to EFEMP1's in vivo suppression of any alteration of subpopulation equilibrium when tumor onset conditions were changed (see Figure 5), EFEMP1-transduced U251-NS showed a similar increase of the Chr7 copy score as seen for that of empty-Vector-transduced cells in response to a change of culture conditions, EFEMP1 overexpression in U251-NS also led to maintaining a large proportion of near tetraploid cells in the culture, with a majority of cells carrying 4 copies of Chr7. Here, EFEMP1 is linked to neoplasm.