Immunofluorescence staining of CDX2 in Ascl2-deficient CRC cells confirmed increased numbers of CDX2-positive cells, as well as increased CDX2 staining intensity in both shRNA-Ascl2/HT-29 and shRNA-Ascl2/LS174T cells when compared with control cells (Figure 2E-2F). Here, ASCL2 is linked to colorectal carcinoma.