PTEN and melanoma: Conversely, small interfering RNA (siRNA)-mediated PTEN knockdown in PTENwt human Lyse melanoma cells, as shown by western blot analysis (Supplementary Fig. 1c), resulted in increased translocation of β-catenin into the nucleus from 40% compared with 2% in control cells (Fig. 1e–h and Supplementary Fig. 1d).