Immunoreactivity in the Western blots (Fig 2A) corroborate the iTRAQ quantitation and show that the primary tumor tissues, relative to the control, contain increased amounts of macrophage migration inhibitory factor, glyceraldehyde-3-phosphate dehydrogenase, glutathione S-transferase omega 1, and lactate dehydrogenase A and decreased amounts of A-kinase anchor protein 12, C9, metalloproteinase inhibitor 3, and vitronectin. Here, AKAP12 is linked to neoplasm.