The present findings, in addition to corroborating a recent study that showed a decrease in ChAT expression density in lamina X ChIns in the SOD1 mouse [19], further support the fact that motoneurons are not the only neurons to degenerate in the spinal cord of SOD1 mice and that spinal interneurons are also targeted by apoptotic processes in this ALS model. This evidence concerns the gene CHAT and amyotrophic lateral sclerosis.