To confirm the immunohistochemical finding and determine whether ANO1 is also upregulated in different pathological types of lung cancer cell lines, we selected and tested 6 different cell lines that include the 2BS cells for normal human lung cell line, GLC82 and Calu-3 cells for adenocarcinoma, NCI-H520 cells for squamous cell carcinoma, and A549 and H1299 cells for unconfirmed type of lung cancer (Fig 2). The gene discussed is ANO1; the disease is lung cancer.