Compared with the sham-operation group, the BDL group of WT and miR-29aTg mice had increased GFAP protein expression (p < 0.001 and p = 0.003, respectively).(Fig 2) Moreover, miR-29a overexpression significantly downregulated GFAP immunoreactivity in the miR-29aTg mice with cholestasis compared with the WT littermates (p < 0.001). The gene discussed is GFAP; the disease is cholestasis.