To further explore the molecular mechanism underlying the induction of glioma invasion by RTVP-1 we performed a pull-down assay using His-tagged RTVP-1 and glioma cell lysates followed by a mass spectrometry analysis and identified the key actin regulator N-WASP as a novel interacting protein of RTVP-1. Here, GLIPR1 is linked to central nervous system cancer.